2022

What makes nanotechnologies applied to agriculture green?

BARTOLUCCI, C. ; ANTONACCI, A. ; SCOGNAMIGLIO, V. ; FRACETO, L. F. What makes nanotechnologies applied to agriculture green? Nano Today 43, 101389 (2022).

Full publication: https://doi.org/10.1016/j.nantod.2022.101389

Nano-pesticides: the lunch-box principle-deadly goodies (semio-chemical functionalised nanoparticles that deliver pesticide only to target species)

AMORIM, M. J.; FRACETO, L. F.; SCOTT-FORDSMAND, J. J. Nano-pesticides: the lunch-box principle-deadly goodies (semio-chemical functionalised nanoparticles that deliver pesticide only to target species). JOURNAL OF NANOBIOTECHNOLOGY 20, 13, 2022.

Full publication: https://doi.org/10.1186/s12951-021-01216-5

Biopolymer/Nanocomposite Hybrid Materials as Potential Strategy to Remove Pesticides in Water: Occurrence and Perspectives

Baigorria, E.; Fraceto, L.F. Biopolymer/Nanocomposite Hybrid Materials as Potential Strategy to Remove Pesticides in Water: Occurrence and Perspectives. ADVANCED SUSTAINABLE SYSTEMS, 6, 2100243 (2022).

Full publication: https://doi.org/10.1002/adsu.202100243

2021

Clove oil-loaded zein nanoparticles as potential bioinsecticide agent with low toxicity

Saraiva, N.R.; Roncato, J.F.F.; Pascoli, M.; Souza, M.F.M.; Windeberg Jr.; F.; Rossato, C.P.; Soares, J.J.; Denardin, L.G.; Puntel, R.L.; Zimmer, K.R.; Fraceto, L.F.; Avila, D.S. Clove oil-loaded zein nanoparticles as potential bioinsecticide agent with low toxicity. Sustainable Chemistry and Pharmacy 24, 100554 (2021).

Full publication: https://doi.org/10.1016/j.scp.2021.100554

Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions

Mujtaba, M.; Wang, D.; Carvalho, L.B.; Oliveira, J.L.; Pereira, A.E.S.; Sharif, R.; Jogaiah, S.; Paidi, M. K.; Wang, L.; Ali, Q.; Fraceto, L.F. Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions. ACS Agricultural Science and Technology 1, 5 417-435 (2021)

Full publication: https://doi.org/10.1021/acsagscitech.1c00146

Foliar absorption and field herbicidal studies of atrazine-loaded polymeric nanoparticles

Takeshita, V.; Sousa, B.T.; Preisler, A.C.; Carvalho, L.B.; Pereira, A.E.S.; Tornisielo, V.L.; Dalazen, G.; Oliveira, H.C.; Fraceto, L.F. Foliar absorption and field herbicidal studies of atrazine-loaded polymeric nanoparticles. Journal of Hazardous Materials 418, 126350 (2021).

Full publication: https://doi.org/10.1016/j.jhazmat.2021.126350

Ecotoxicity evaluation of polymeric nanoparticles loaded with ascorbic acid for fish nutrition in aquaculture

Luis, A.I.S.; Campos, E.V.R.; Oliveira, J.L.; Vallim, J.H.; Proença, P.L.F.; Castanha, R.F.; Casto, V.L.S.S.; Fraceto, L.F. Ecotoxicity evaluation of polymeric nanoparticles loaded with ascorbic acid for fish nutrition in aquaculture. Journal of Nanobiotechnology 19, 163 (2021).

Full publication: https://doi.org/10.1186/s12951-021-00910-8

Zein based-nanoparticles loaded botanical pesticides in pest control: an enzyme stimuli-responsive approach aiming sustainable agriculture

Monteiro, R.A.; Camara, M.C.; Oliveira, J.L.; Campos, E.V.R.; Carvalho, L.B.; Proença, P.L.F.; Guilger-Casagrande, M.; Lima, R.; Nascimento, J.; Gonçalves, K.C.; Polanczyk, R.A.; Fraceto, L.F. Zein based-nanoparticles loaded botanical pesticides in pest control: An enzyme stimuli-responsive approach aiming sustainable agriculture. Journal of Hazardous Materials 417, 126004 (2021).

Full publication: https://doi.org/10.1016/j.jhazmat.2021.126004

Encapsulation strategies for Bacillus thuringiensis: From now to the Future

Oliveira, J.L.; Fraceto, L.F.; Bravo, A.; Polanczyk, R.A. Encapsulation strategies for Bacillus thuringiensis: from now to the future. Journal of the Agricultural and Food Chemistry 69, 4564-4577 (2021).

Full publication: https://doi.org/10.1021/acs.jafc.0c07118

Trends in polymers networks applied to the removal of aqueous pollutants: A review

Baigorria, E.; Galhardi, J.A.; Fraceto, L.F. Trends in polymers networks applied to the removal of aqueous pollutants: A review. Journal of Cleaner Production 295, 126451 (2021).

Full publication: https://doi.org/10.1016/j.jclepro.2021.126451

Use of nontarget organism Chironomus sancticaroli to study the toxic effects of nanoatrazine

Albuquerque, F.P.; Oliveira, J.L.; Machado, L.S.; Richardi, V.S.; Silva, M.A.N.; Pompeo, M.L.; Fraceto, L.F.; Carlos, V.M. use of nontarget organism Chironomus sancticaroli to study the toxic effects of nanoatrazine. Ecotoxicology 30, 733-750 (2021)

Full publication: https://doi.org/10.1007/s10646-021-02400-x

Influence of the capping of biogenic silver nanoparticles on their toxicity and mechanism of action towards Sclerotinia sclerotiorum

Guilger-Casagrande; M.; Germano-Costa, T.; Bilesky-Jose, N.;Pasquoto-Stigliani, T.; Carvalho, L.; Fraceto, L.F.; Lima, R. Influence of the capping of biogenic silver nanoparticles on their toxicity and mechanism of action towards Sclerotinia sclerotiorum. Journal of Nanobiotechnology, 19, 53 (2021).

Full publication: https://doi.org/10.1186/s12951-021-00797-5

Biogenic α-Fe2O3 Nanoparticles Enhance the Biological Activity of Trichoderma against the Plant Pathogen Sclerotinia sclerotiorum

Bilesky-José, N.; Maruyama, C.; Germano-Costa, T.; Campos, E.; Carvalho, L.; Grillo, R.; Fraceto, L.F.; Lima, R. Biogenic α-Fe2O3 Nanoparticles Enhance the Biological Activity of Trichoderma against the Plant Pathogen Sclerotinia sclerotiorum. ACS Sustainable Chemistry & Engineering 9, 4, 1669-1683 (2021).

Full publication: https://doi.org/10.1021/acssuschemeng.0c07349

Sericin based nanoformulations: a comprehensive review on molecular mechanisms of interaction with organisms to biological applications

Das, G.; Shin, H.S.; Campos, E.V.R.; Fraceto, L.F.; Rodriguez-Torres, M. P.; Mariano, K.C.; Araujo, D.R.; Fernández-Luqueno, F.; Grillo, R.; Patra, J.K. Sericin based nanoformulations: a comprehensive review on molecular mechanisms of interaction with organisms to biological applications. Journal of Nanobiotechnology, 19, 30 (2021).

Full publication: https://doi.org/10.1186/s12951-021-00774-y

Nanotechnology Potential in Seed Priming for Sustainable Agriculture

Pereira, A.E.S.; Oliveira, H.C.; Fraceto, L.F.; Santaella, C. Nanotechology Potential in Seed Priming for Sustainable Agriculture. Nanomaterials 11, p.267 (2021).

Full publication: https://doi.org/10.3390/nano11020267

Enzyme Stimuli–Responsive Nanoparticles for Bioinsecticides: An Emerging Approach for Uses in Crop Protection

Camara, M.C.; Monteiro, R.A.; Carvalho, L.B.; Oliveira, J.L.; Fraceto, L.F. Enzyme Stimuli–Responsive Nanoparticles for Bioinsecticides: An Emerging Approach for Uses in Crop Protection. ACS Sustainable Chemistry & Engineering 9,106-112 (2021).

Full publication: https://doi.org/10.1021/acssuschemeng.0c08054

Ecotoxicological and regulatory aspects of environmental sustainability of nanopesticides

Grillo, R.; Fraceto, L.F.; Amorim, M.J.B.; Scott-Fordsmand, J.J.; Schoonjans, R.; C. Qasim. Ecotoxicological and regulatory aspects of environmental sustainability of nanopesticides.Journal of Hazardous Materials 404, Part A, 124148 (2021).

Full publication: https://doi.org/10.1016/j.jhazmat.2020.124148

2020

Fabrication and Characterization of a Novel Herbicide Delivery System with Magnetic Collectability and Its Phytotoxic Effect on Photosystem II of Aquatic Macrophyte

Forini,M.M.L; Antunes, D.R.; Cavalcante, L.A.F.; Pontes, M.S.; Biscalchim, E.R.; Sanches, A.O.; Santiago,E.F.; Fraceto, L.F. and Grillo, R. Fabrication and Characterization of a Novel Herbicide Delivery System with Magnetic Collectability and Its Phytotoxic Effect on Photosystem II of Aquatic Macrophyte. J. Agric. Food Chem. 68, 40, 11105–11113(2020).

Full publication: https://doi.org/ 10.1021/acs.jafc.0c03645

Soil Enzyme Activities as an Integral Part of the Environmental Risk Assessment of Nanopesticides

Galhardi, J.A.; Fraceto, L.F.; Wilkinson, K.J.; Ghoshal, G. Soil Enzyme Activities as an Integral Part of the Environmental Risk Assessment of Nanopesticides J. Agric. Food Chem. 68, 8514−8516 (2020).

Full publication: https://doi.org/10.1021/acs.jafc.0c04344

Localization of Coated Iron Oxide (Fe3O4) Nanoparticles on Tomato Seeds and Their Effects on Growth

Lau, E.C.H.T.; Carvalho, L.B.; Pereira, A.E.S.; Montanha, G.S.; Corrêa, C.G.; Carvalho, H.W.P.; Ganin, A.Y.; Fraceto, L.F.; Yiu, H.P. Localization of Coated Iron Oxide (Fe3O4) Nanoparticles on Tomato Seeds and Their Effects on Growth. ACS Appl. Bio Mater 3, 4109-4117 (2020).

Full publication: https://doi.org/10.1021/acsabm.0c00216

Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish Pathogens

Luis, A.I.; Campos, E.V.R.; Oliveira, J.L.; Guilger-Casagrande, M.; Lima, R.; Castanha, R.F.; Castro, V.L.S.S.; Fraceto, L.F.Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish Pathogens. ACS Omega 5 (25), 15557–15566 (2020).

Full publication: https://doi.org/10.1021/acsomega.0c01716

Effectiveness of nanoatrazine in post-emergent control of the tolerant weed Digitaria insularis

Sousa, B.T.; Pereira, A.E.S.; Fraceto, L.F.; Oliveira, H.C.; Dalazen, G. Effectiveness of nanoatrazine in post-emergent control of the tolerant weed Digitaria insularis. Journal of Plant Protection Research 60(2), 185–192 (2020).

Full publication: https://doi.org/10.24425/jppr.2020.133311

BOOK – Advances in Nano-Fertilizers and Nano-Pesticides in Agriculture – A Smart Delivery System for Crop Improvement

Jogaiah, S.; Singh, H.B.; Fraceto, L.F.; Lima, R. (Editors) Advances in Nano-Fertilizers and Nano-Pesticides in Agriculture – A Smart Delivery System for Crop Improvement, Elsevier, p.668, 1st Edition, 2020.ISBN 978-0-128-20092- 6.

Full publication: https://www.elsevier.com/books/advances-in-nano-fertilizers-and-nano-pesticides-in-agriculture/jogaiah/978-0-12-820092-6

BOOK – Nanopesticides: From Research And Development To Mechanisms Of Action And Sustainable Use In Agriculture

Fraceto, L.F.; Castro, V.L; Grillo, R.; Avila, D.; Caixeta Oliveira, H.; Lima, R. (Editors) From Research And Development To Mechanisms Of Action And Sustainable Use In Agriculture, Springer, p. 353. ISBN 978-3-030-44872-1.

Full publication: https://doi.org/10.1007/978-3-030-44873-8

BOOK – Green Nanoparticles – Synthesis and Biomedical Applications

Patra, J. K.; Fraceto, L.F.; Das, G.; Campos, E.V.R. (Editors) Green Nanoparticles – Synthesis and Biomedical Applications, Springer, p 395 (2020). ISBN 978-3-030-39245-1

Full publication: https://doi.org/10.1007/978-3-030-39246-8

Chitosan-based delivery systems for plants: A brief overview of recent advances and future directions

Mujtaba, M.; Kawar, M.K.; Camara, M.C.; Carvalho, L.B.; Fraceto, L.F.; Morsi, R.E.; Elsabee, M.Z.; Kaya, M.; Labidi, J.; Ullah, H.; Wang, D. Chitosan-based delivery systems for plants: A brief overview of recent advances and future directions. International Journal of Biological Macromolecules, 154, 683-697 (2020).

Full publication: https://doi.org/10.1016/j.ijbiomac.2020.03.128

Encapsulation of Trichoderma harzianum Preserves Enzymatic Activity and Enhances the Potential for Biological Control

Maruyama, C.R.; Bilesky-José, N.; de Lima, R.; Fraceto, L.F. Encapsulation of Trichoderma harzianum Preserves Enzymatic Activity and Enhances the Potential for Biological Control. Front. Bioeng. Biotechnol. 8, 225 (2020).

Full publication: https://doi.org/10.3389/fbioe.2020.00225

Green nanomaterials fostering agrifood sustainability

Bartolucci, C.; Antonacci, A.; Arduini, F.; Moscone, D.; Fraceto, L.; Campos, E.; Attaallah, R.; Amine, A.; Zanardi, C.; Cubillana-Aguilera, L.M.; Santander, J.M.P.; Scognamiglio V. Green nanomaterials fostering agrifood sustainability. TrAC Trends in Analytical Chemistry, 125, 115840, 2020.

Full publication: https://doi.org/10.1016/j.trac.2020.115840

The potential of nanobiopesticide based on zein nanoparticles and neem oil for enhanced control of agricultural pests

Pascoli, M.; Albuquerque, F.P.; Calzavara, A.K.; Tinoco-Nunes, B.; Oliveira, W.H.C.; Gonçalvez, K.C.; Polanczyk, R.A.; Vechia, J.F.D.; Matos, S.T.S.; Andrade, D.J.; Oliveira, H. C.; Souza-Neto, J.A.; Fraceto, L.F. The potential of nanobiopesticide based on zein nanoparticles and neem oil for enhanced control of agricultural pests. Journal of Pest Science 93, 793-806 (2020).

Full publication: https://doi.org/10.1007/s10340-020-01194-x

Hydrogels Containing Botanical Repellents Encapsulated in Zein Nanoparticles for Crop Protection

de Oliveira, J.L.; Campos, E.V.R.; Camara, M.C.; Vechia, J.F.D.; Matos, S.T.S.; Andrade, D.J.; Gonçalves, K.C.; Nascimento, J.; Polanczyk, R.; Araujo, D.R.; Fraceto, L.F. Hydrogels Containing Botanical Repellents Encapsulated in Zein Nanoparticles for Crop Protection. ACS Appl. Nano Mater. 3, 207-217 (2020).

Full publication: https://doi.org/10.1021/acsanm.9b01917

Interference of goethite in the effects of glyphosate and Roundup® on ZFL cell line

Silva, N.D.G.; Carneiro, C.E.A.; Campos, E.V.R.; Oliveira, J.L.; Risso, W.E.; Fraceto, L.F.; Zaia, D.A.M.; Martinez, C.B.R. Interference of goethite in the effects of glyphosate and Roundup® on ZFL cell line. Toxicology in vitro 65, 104755 (2020).

Full publication: https://doi.org/10.1016/j.tiv.2019.104755

Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: A comparative approach

Tesser, M.E; Paula, A.A.; Risso, W.E.; Monteiro, R.A.; Pereira, A.E.S.; Fraceto, L.F.; Martinez, C.B.R. Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: A comparative approach. Science of the Total Envioronment 704, 135332 (2020).

Full publication: https://doi.org/10.1016/j.scitotenv.2019.135332

Atrazine nanoencapsulation improves pre-emergence herbicidal activity against Bidens pilosa without enhancing long-term residual effect on Glycine max

Preisler, A.C.; Pereira, A.E.S.; Campos, E.V.R; Dalazen, G.; Fraceto, L.F.; Oliveira, H.C. Atrazine nanoencapsulation improves pre-emergence herbicidal activity against Bidens pilosa without enhancing long-term residual effect on Glycine max. Pest Manag. Sci 76, 141-149 (2020)

Full publication: https://doi.org/10.1002/ps.5482

An overview of the potential impacts of atrazine in aquatic environments: Perspectives for tailored solutions based on nanotechnology

Albuquerque, F.P.; Oliveira, J.L.; Moschini-Carlos, V.; Fraceto, L.F. Na overview of the potential impacts of atrazine in aquatic environments: Perspectives for tailored solutions based on nanotechnology. Science of the Total Environment 700, 134868, (2020).

Full publication: https://doi.org/10.1016/j.scitotenv.2019.134868

2019

Capítulo de Livro – Uso de micro e nanotecnologia com Trichoderma

Fraceto, L.F.; Maruyama, C.R.; Guilger-Casagrande, M.; Bilesly-José, N.; Lima, R. Uso de micro e nanotecnologia com Trichoderma, em Trichoderma:Uso na Agricultura, Editora da Embrapa, 2019, Capítulo 10, p. 297-314.

Full publication: http://ainfo.cnptia.embrapa.br/digital/bitstream/item/208230/1/livro-trichoderma-online-06.01.20.pdf

Biosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum

Guilger-Casagrande, M.; Pasquioto-Stigliani, T.; Fraceto, L.F.; Lima, R. Biosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum. Scientific Reports 9, 14351, (2019).

Full publication: https://doi.org/10.1038/s41598-019-50871-0

Development of stimuli-responsive nano-based pesticides: emerging opportunities for agriculture

Camara, M.C.; Campos, E.V.R.; Monteiro, R.A.; Pereira, A.E.S.; Proença, P.L.F.; Fraceto, L.F. Development of stimuli-responsive nano-based pesticides: emerging opportunities for agriculture. Journal of Nanobiotechnology 17, 100, (2019).

Full publication: https://doi.org/10.1186/s12951-019-0533-8

Book chapter – Potential use of polymeric particles for the regulation of plant growth

Pereira, A.E.S.; Sousa, B.T.; Iglesias, M.J.; Alvarez, V.A.; Casalongué, C. A.; Oliveira, H.C.; Fraceto, L.F. Potential use of polymeric particles for the regulation fo plant growth, in T.J.Gutierrez (ed.), Polymers for Agri-food Applications, Springer Nature Switzerland, 2019, Chapter 4, p.45-66

Full publication:  https://doi.org/10.1007/978-3-030-19416-1_4

Pyrethrum extract encapsulated in nanoparticles: toxicity studies based on genotoxicity and hematological effects in bullfrog tadpoles

Oliveira C.R.; Garcia, T.; Franco-Belussi, L.; Salla, R.; Melo, N.F.S.; Irazusta, S.; Costa, M.J.; Zacarin, E.C.M.S.; Fraceto, L.F. Pyrethrum extract encapsulated in nanoparticles: toxicity studies based on genotoxicity and hematological effects in bullfrog tadpoles. Environmental Pollution 253, 1009-1020 (2019)

Full publication: https://doi.org/10.1016/j.envpol.2019.07.037

Nanopesticide based on botanical insecticide pyrethrum and its potential effects on honeybees

Oliveira C.R.; Domingues, C.E.C.; Melo, N.F.S.; Roat, T.C.; Malaspina, O.; Jones-Costa, M.; Silva-Zacarin, E.C.M; Fraceto, L.F. Nanopesticide based on botanical insecticide pyrethrum and its potential effects on honeybees. Chemosphere 236, 124282 (2019).

Full publication: https://doi.org/10.1016/j.chemosphere.2019.07.013

Book chapter – Bio-Based Nanoemulsion Formulations Applicable in Agriculture, Medicine, and Food Industry

Jampílek, J.; Kráľová, K.; Campos, E.V.R; Fraceto, L.F. Bio-Based Nanoemulsion Formulations Applicable in Agriculture, Medicine, and Food Industry, in R. Prasad et al. (eds.), Nanobiotechnology in Bioformulations, Nanotechnology in the Life Sciences, Springer Nature Switzerland, 2019, Chapter 2, p.34-67

Full publication:  https://doi.org/10.1007/978-3-030-17061-5_2

Association of zein nanoparticles with botanical compounds for effective pest control systems

Oliveira, J.L.; Campos, E.V.R.; Germano-Costa, T.; Lima, R.; Della Vechia, J.F.; Soares, S.T.; Andrade, D.J.; Gonçalves, K.C.; Nascimento, J.; Polanczyk, R.; Fraceto, L.F. Association of zein nanoparticles with botanical compounds for effective pest control systems. Pest. Manag. Sci 75, 1855-1865 (2019).

Full publication: https://doi.org/10.1002/ps.5338

On the safety of nanoformulations to non-target soil invertebrates – an atrazine case study

Gomes, S.I.L.; Scott-Fordsmand, J.J.; Campos, E.V.R; Grillo, R.; Fraceto, L.F.; Amorim, M.J.B.  On the safety of nanoformulations to non-target soil invertebrates – an atrazine case study. Environ. Sci.: Nano 6, 1950–1958 (2019).

Full publication: https://doi.org/10.1039/c9en00242a

An eco-designed paper-based algal biosensor for nanoformulated herbicide optical detection

Scognamiglio, V.; Antonacci, A.; Arduini, F.; Moscone, D.; Campos, E.V.R.; Fraceto, L.F.; Palleschi, G. An eco-designed paper-based algal biosensor for nanoformulated herbicide optical detection. Journal of Hazardous Materials 373, 483-492 (2019)

Full publication: https://doi.org/10.1016/j.jhazmat.2019.03.082

Polymeric nanoparticles as an alternative for application of gibberellic acid in sustainable agriculture: a field study

Pereira, A.E.S.; Oliveira, H.C.; Fraceto, L.F. Polymeric nanoparticles as an alternative for application of gibberellic acid in sustainable agriculture: a field study. Scientific Reports 9, 7135 (2019)

Full publication: https://doi.org/10.1038/s41598-019-43494-y

Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: An ecotoxicological perspective

Pascoli, M.; Jacques, M.T.; Agarrayua, D.A.; Avila, D.S.; Lima, R.; Fraceto, L.F. Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: An ecotoxicological perspective. Science of the Total Environment 677, 57-67 (2019)

Full publication: https://doi.org/10.1016/j.scitotenv.2019.04.345

A Mechanistic View of Interactions of a Nanoherbicide with Target Organism

Bombo, A.B.; Pereira, A.E.S.; Lusa, M.G.; Oliveira, E.M.; Oliveira, J.L.; Campos, E.V.R.; de Jesus, M.B.; Oliveira, H.C.; Fraceto, L.F.; Mayer, J.L.S. A Mechanistic View of Interactions of a Nanoherbicide with Target Organism, Journal Agricultural and Food Chemistry, 67, 16, 4453–4462 (2019)

Full publication: https://doi.org/10.1021/acs.jafc.9b00806

Can atrazine loaded nanocapsules reduce the toxic effects of this herbicide on the fish Prochilodus lineatus? A multibiomarker approach

Andrade, L.L; Pereira, A.E.S.; Fraceto, L.F.; Martinez, C.B.R. Can atrazine loaded nanocapsules reduce toxic effects of this herbicide on the fish Prochilodus lineatus? A multibiomarker approach. Science of the Total Environment, 663, 1, 548-559. (2019)

Full publication: https://doi.org/10.1016/j.scitotenv.2019.01.380